Evaporation Effects on Measurement Stability – EkkO – Spectroscopy – Application Note 29

Abstract

In this note, we address the effects of solvent evaporation in well plate applications using the EKKO™ CD Microplate Reader with CD measurements of (+)-camphorsulfonic acid (CSA), (-)-pantolactone (PL), and bovine serum albumin (BSA) with purposely extended exposure to and protection from the environment to ensure the presence and absence of the evaporative loss of solvent.

Introduction

Circular dichroism (CD), the commonly used technique for chiral analysis, refers to the differential absorption between left and right circularly polarized light. It is often used for assigning the secondary structures of proteins and determining enantiomeric purities in asymmetric syntheses, both of which require the ability to do the measurements in a high-throughput fashion.

The primary advantage of the EKKO™ CD Microplate Reader is its speed resulting from the use of well plates allowing for the highest throughput possible. It accomplishes this by
turning the light path from the horizontal to vertical, allowing for the use of a computer-controlled XY stage so that CD signals are read directly from a well plate.

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