Millisecond-to-Minute Protein Folding/Misfolding Events Monitored by FTIR Spectroscopy

Authors: Heinz Fabian, Dieter Naumann
Ref: Biological and Medical Physics, Biomedical Engineering 2012, pp 53-89

Abstract

The adaptation of conventional mixing or temperature-jump technologies to the specific requirements of time-resolved FTIR spectroscopy enables to explore protein folding/misfolding events on the millisecond to minute timescale by monitoring spectral changes in several spectral windows simultaneously. We present a description of experimental setups and representative results on different proteins, which illustrate the advantages and limitations of the FTIR approaches.

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