Protein folding and Chevron plots – SFM Series – Stopped-flow – AN36

Protein folding is the physical process by which a protein chain acquires its native 3-dimensional structure in the cell, a conformation that is usually biologically functional. For some reasons, proteins do not always fold as they should and misfold. This dysfunction is the source of several degenerative diseases such as Creutzfeldt-Jakob and Alzheimer. So, it is essential to truly understand the folding process in the quest of future drugs.
This application note demonstrates how to get the very best out of your stopped-flow instrument in order to measure protein stability. You will learn how to measure the chemical denaturation midpoint of a protein and how to build easily and rapidly Chevron plots taking advantage of the independent stepping motors of the SFM stopped-flow. Concentration dependence studies are achieved without manual dilution to reduce human handling errors and experimental time.