RK-Spectro Technical Note 31: Determination of your experimental dead time in absorbance mode

Introduction

A Technical Note related to the determination of experimental dead time. The dead time of the experiment is the age of the solution at the observation point, in other words, it represents the time for the mixed solution to go from the center of the last mixer to the observation point. The dead time depends on many factors besides simply the total flow rate and the cuvette volume. A slight difference between the estimated dead time (given by the Biokine software) and the real dead time may be observed. This is due to the hydrodynamics phenomenon….