Stopped-flow in cryogenic conditions – SFM-2000 series – Rapid kinetics – Application Note 25

Abstract

This application note describes the use of the cryo accessory combined to an external cryostat which extends the temperature range of the SFM to -90°C allowing for these lower temperature regime reactions to be observed. The reaction between 2,4 dinitrophenyl acetate (DNPA) and sodium methoxide in organic solvents is used to illustrate the use of this accessory in a SFM equipped with the appropriate o-rings.

Introduction

The SFM-series of stopped-flows are temperature regulated using a circulating water bath. In their standard configuration, the stopped-flows may be operated from -20°C to +85°C, offering more than a 100 degrees temperature range to the user. However, some applications require lower temperatures to slow down the reactions enough to enable the observation of the intermediates and final products optically. This application note describes the use of the CS90°C accessory which extends the temperature range of the SFM to -90°C allowing for these lower temperature regime reactions to be observed. The reaction between 2,4 dinitrophenyl acetate (DNPA) and sodium methoxide in organic solvents is used to illustrate the use of this accessory in an SFM equipped with the appropriate O-rings.

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