Well Plate Optical Characteristics Variation Effects on CD Measurements – EkkO – Spectroscopy – Application Note 34

Abstract

In this technical note, we address this possible effect on the data obtained with the EKKO  CD Microplate Reader with CD measurements of Insulin in two different solid fused silica 96 well plates obtained from Hellma. We show qualitative and quantitative CD measurements can be done at all wavelength using different operation modes and well plates.

Introduction

The differential absorption between left and right circularly polarized light is a commonly used technique. Circular dichroism (CD) is often used for assigning the secondary structures of proteins and determining enantiomeric purities in asymmetric syntheses, both of which benefit from the ability to do the measurements in a highthroughput fashion 1,2.

The primary advantage of the EKKO™ CD Microplate Reader is its speed resulting from the use of well plates allowing for the highest throughput possible. It accomplishes this by turning the light path from the horizontal to vertical, allowing for a computer-controlled XY stage so that CD signals are read directly from a well plate…

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