Micro-volume Stopped-flow

Micro-volume operation is now a real requirement for stopped-flow users. Biochemists and biologists often spend days or weeks in order to prepare and purify a few hundred microliters of precious sample.

It therefore makes sense that their time will be better spent, if this process is made as efficient as possible.

Previously, the idea of running a complete series of experiments with only 100µl of solution was considered a pipe dream, as such volumes were necessary just for priming the instrument.

100 µl stock, 10 shots, 1 ms dead time

The reduction of sample consumption in stopped-flow instruments was our driving motivation for the development of the µSFM. Not a single drop of precious liquid is wasted in the stopped-flow or in the storage vial. With only a 100 µl stock solution, the user has enough solution to carry out 10 replicates. And this could even be extended to 25 replicates when carrying out refolding/folding experiments! This performance is done using our standard microcuvette so, there is no loss of sensitivity and only 1ms dead time.

Mixing precision and modularity

The µSFM is a two-syringe stopped-flow system. Each syringe is driven by an independent stepping motor so the mixing ratio can be freely changed in Bio-Kine software from 1:1 to 1:9. The combination of high-precision motors with the precision of micro-volume glass syringes allows the user to inject a few µl of precious samples at each experiment. A series of experiments are possible with only 50µl of stock solution and without compromising on sensitivity. Minimum injection volume can be reduced down to 3µl in asymmetric mixing conditions.

The µSFM is a dedicated single mixing stopped-flow, optimized for absorbance, fluorescence, 90° light scattering, and X-ray kinetics. Users seeking increased modularity should consider our premium stopped-flow range.

10 years warranty

Bio-Logic offers a 10 years warranty on the stepping motors. Our 10 years warranty reflects our confidence in the high quality of our design, engineering, and product performance.  Bio-Logic instruments are designed to be future-proof. They are instruments that will withstand the tests of time, the rigors of the laboratory and the ever-increasing demands of your research.

Bio-Kine is user-friendly software that controls the stopped-flow, the detection device, follows the acquisition, and analyzes data.

Single mixing applications

Two levels of operation are provided for mixer control. The first level is designed for rapid and easy experiment design. The user sets the mixing ratio, the size of the cuvette and the volume of samples. Color coded windows display calculated values and alert the user to out of range conditions. The estimated dead time is automatically displayed, and a pre-trigger is also available to be sure the stationary state is reached accurately. This interface is ideal for routine mixing designs, occasional users, or students.

Specifications

• Optimized for micro-volume applications
• 10 valuable shots from a 100 µl stock solution
• Minimum injection volume: 3µl
• 2 independently controlled syringes
• Mixing ratio from 1:1 to 1:9
• User-selectable mixing ratio
• Full solvent compatibility
• Deadtime: 1ms
• Compatible with all MOS spectrometers
• Temperature range: 0 to +50°C
• USB communication