FT-IR Stopped-flow - BioLogic
Stopped-flow /quench flow

FT-IR Stopped-flow.

A valuable tool for evaluating reaction rates in Infra-Red regions.

Provides structural information in terms of bonds and mobility, the accessory can be used for infrared kinetics.

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Provides structural information in terms of bonds and mobility, the accessory can be used for infrared kinetics.

Improvement in Fourier Transform Infra-Red (FT-IR) technology and the use of step scan acquisition have made coupling the FT-IR with stopped-flow instruments a valuable tool for evaluating reaction rates in Infra-Red regions. FT-IR spectroscopy provides structural information in terms of bonds and mobility. It can be a very useful accessory to study infrared kinetics in the amide I region of a protein’s vibrational spectrum.

Compatible with most FT-IR spectrometers

The FT-IR accessory is usually attached to stopped-flow using an umbilical connector so only the observation cuvette is fitted into FT-IR sample compartment. The FT-IR flow cell is mounted in a holder compatible with most FT-IR instruments.

 

The flow cell uses CaF2 windows and user can freely select light paths from 15 μm to 500 μm by changing a spacer between windows. Depending on the light path the total flow rate may be limited to 1 ml/s. The dead time under these conditions is determined by the acquisition speed of the detector.

FT-IR accessory includes:

  • FT-IR flow cell holder with built-in Berger Ball mixer,
  • Set of CaF2 windows,
  • Set of spacers,
  • Direct exit tube.

Compatible with  dual-comb spectrometers

The FT-IR accessory can also be installed in the sample compartment of a dual-comb spectrometer to benefit from the high acquisition speed, the enhanced signal to noise ratio and the wavelength resolution of such technologies.

Specifications  

Window material: CaF2

Lightpath:15 μm, 25 μm, 50 μm, 100 μm, 200 μm, 500 μm

Dead time :(with 100 μm spacer and 3 ml/s flow rate)     6 ms